Home > Search Results: keywords:"glycopeptide separation"
Search:
Search Tips :: Advanced Search
Search collections:
Sort by: Display results: Output format:
National Repository of Grey Literature 1 records found  Search took 0.00 seconds. 
Development of chomatography-mass spectrometry technique for separation and identification of glycopeptides applicable in clinical practice
 Petroušek, Filip ; Ječmen, Tomáš (advisor) ; Kukačka, Zdeněk (referee)
Glycoproteins are commonly found in organisms and play an important role in both physiological and pathological conditions. In glycoproteins, different glycans may be present at the same glycosylation site and altered glycosylation of proteins may be a manifestation of disease. As a result, they can be used as markers in diagnostics. For the analysis of glycoproteins, their cleavage into glycopeptides, which are then identified, is often used. Liquid chromatography coupled to mass spectrometry is a widely used method for the study of glycopeptides and hydrophilic interaction chromatography (HILIC) can be used for the separation of glycopeptides. This type of chromatography allows separation of glycopeptides based on the properties of both the peptide and the bound glycan. The first aim of this work was to determine the extent to which the charge of the peptide backbone of glycopeptides affects their separation by HILIC. Penta-HILIC and HILIC-B columns were used for this purpose. Penta-HILIC contains a polyhydroxyl stationary phase, while the stationary phase of HILIC-B consists of silica gel modified with positively charged aminopropyl groups. IgG1 and IgG2 glycopeptides were separated on both columns. To determine the extent of the effect of charge and hydrophobicity during separation on HILIC-B,...
Detailed record

Interested in being notified about new results for this query?
Subscribe to the RSS feed.
Digital Repository :: Search :: Submit :: Personalize :: Help
Powered by Invenio v1.1.2
Maintained by admin.invenio@techlib.cz
This site is also available in the following languages:
Česky  English